Consultation
Assay Design

Clarity, Where It Matters. When something doesn’t work, the answer is rarely obvious.

We evaluate the full system to identify what’s limiting performance.

Our Approach Function First

Built from experience at the bench, where outcomes—not specifications—define success.

We validate function. We define conditions. We deliver what works.

Assay design and optimization
We help refine conditions to ensure your assay is sensitive, reliable, and suited to your specific question.
Troubleshooting with context
When something isn’t working, we look across variables—protein quality, buffer conditions, setup—to identify what may be limiting performance.
Protein performance validation
We assess whether your protein is behaving as expected within your assay, and adjust conditions where needed.
Guidance across disciplines
For teams working outside traditional biochemistry, we provide practical support to make protein-based experiments more accessible and successful.

Assay Design Consulting:

Providing expertise and strategy—helping you design the right experiments and troubleshoot failed data.

Strategic Planning (Before you start):
- Technology Selection: Deciding best assays to use on your sensitivity needs and budget.
- DoE (Design of Experiments): Creating the plan to ensure your assay is robust enough for its intended use.
Technical Troubleshooting (During the work):
- Root Cause Analysis: Investigating why an assay has low activity or is underperforming.
- Data Review: Independently reviewing results and independently auditing raw data from a CRO.
Tech Transfer (End of project):
- Tech Transfer: Supervise transfer of the assay and troubleshooting of technical challenges.

Assay Development:

Phase I: Feasibility & Reagent Sourcing
- Reagent Evaluation: Testing your proteins or sourcing new ones (commercial or custom production).
- Proof-of-Concept: Basic experiments to see if the protein performs specific basic activity.
Phase II: Optimization (The "Development" Phase)
- Matrix Testing: Ensuring the protein works in your assay without interference.
- Parameter Tuning: Optimizing activity through testing various buffer conditions, time and temperature.
Phase III: Validation & Deliverables
- Performance Data: Establishing the Limit of Detection, dynamic range, and reproducibility.
- Transfer Protocol: A detailed Standard Operating Procedure (SOP) and troubleshooting any transfer fails.
- Reagent Kit: Reagents and standards sufficient for your initial study.

$2000 Basic Assay Design Consultation/Additional $200/hr

$4000-$6000 Basic Assay Development/Additional $200/hr