Every protein is different. So is every experiment.

We design, express, and purify proteins aligned to your application

Design → Expression → Purification → Optimization

Nothing generic. Everything personal and intentional.

Custom Protein

Project-focused design
We consider your experimental goals from the outset, helping shape constructs and strategies that are more likely to succeed.
Integration with your system
Whether your work is biochemical, biophysical, or interdisciplinary, we align with your existing platform to ensure compatibility and performance.
Optimization of function
We focus not only on producing protein, but on ensuring enzyme activity and behavior are appropriate for your intended use.
Iterative problem-solving
For challenging targets, we refine conditions step by step—improving expression, solubility, and stability where needed.

A standard production package for expression in E. coli:

Amount< 20 mg, >95% purity, soluble protein <100 kDa single subunit, 2-4 weeks.

Phase I: Molecular Biology (if needed)
- Codon Optimization: Redesigning the gene sequence to match preference for higher expression.
- Gene Synthesis & Sub-cloning: Building the DNA and inserting it into an expression vector (e.g., pET system).
Phase II: Expression Testing (Pilot Study)
- Strain Screening: Testing different strains (e.g., BL21).
- Small-scale expression: Evaluating solubility and yield before full-scale production.
Phase III: Scale-up and Purification
- Large-scale Culture: 1L to 8L of culture.
- Purification: Usually two-step purification (e.g., 1. Affinity for tags, 2. Protein-specific polishing column).
Phase IV: Deliverables & QC
- Purified Protein: In your specified buffer.
- Quality Control Data: SDS-PAGE (purity), Western Blot (identity: anti-tag, specific: Ab tested and provided by client), and a Certificate of Analysis (CoA).
Once the protocol is established, scale-up production can be implemented. $2000, 50-100 mg